Analysis of Val aminoacyl synthetase.

Analysis performed on 2024-09-30 10:31:11

Number of Proteins Analyzed

Initial Filtered Out Final Blast
Bacteria 48049 735 47314 26571
Archaea 3644 161 3483 1423
Vertebrata 1288 502 786 240
Mammals 700 153 547 132

Number of Variants Analyzed:

Protein Length Pathogenic Variants Benign/Unknown Variants
1264 36 958

Domains

Domains
Current Study 1, 278
Guo et al (2010) 2, 218

Results

Mutations in Ancient vs Modern Domains

Current Study

Ancient Domains Modern Domains All Ratio
Number of Residues 987 277 1264 3.563177
Number of Variants 767 227 994 3.378855
Number of Pathogenic Variants 29 7 36 4.142857
Number of Benign/Unknown Variants 738 220 958 3.354545
Fisher exact test 0.839591

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Guo et al (2010)

Ancient Domains Modern Domains All Ratio
Number of Residues 1047 217 1264 4.824885
Number of Variants 806 188 994 4.287234
Number of Pathogenic Variants 29 7 36 4.142857
Number of Benign/Unknown Variants 777 181 958 4.292818
Fisher exact test 1.000000

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Coverage of the alignment and domain locations

Agreement between the current study and Guo et al (2010)

Conservation of the amino acids in modern and ancient domains

# of sites Ancient # sites Modern Mean Ancient (SD) Mean Modern (SD) Median Ancient Median Modern Mann-Whitney U p-value
Current Study 987 277 0.81 (0.01) 0.74 (0.01) 8.373e-01 7.402e-01 167649.50 4.05e-09
Guo et al (2010) 1047 217 0.80 (0.01) 0.74 (0.01) 8.338e-01 7.265e-01 138133.00 2.66e-07

The Mann-Whitney U test was calculated using the conservation scores of the amino acids in ancient and modern domains. The p-value is for the null hypothesis that the conservation scores in ancient domains are greater than in modern domains.

Conservation of the amino acids in modern and ancient domains